ELISA Kit: A Reliable Tool for Accurate Biomolecule Detection
An ELISA kit is a widely used tool in laboratories and medical research for detecting and quantifying biomolecules such as proteins, antibodies, hormones, and pathogens. The enzyme-linked immunosorbent assay (ELISA) technique is known for its high sensitivity and specificity, making it an essential method in diagnostics, immunology, and drug development. How Does an ELISA Kit Work? The ELISA test relies on antigen-antibody interactions to detect the presence of a target molecule in biological samples like blood, serum, or tissue extracts. Enzymes linked to antibodies generate a measurable signal, typically a color change, indicating the presence and concentration of the analyte. The test is highly adaptable, with different formats designed for various applications. Types of ELISA Kits Direct ELISA – Uses a single labeled antibody to detect the target antigen. Indirect ELISA – Involves a primary antibody followed by a secondary enzyme-linked antibody for enhanced sensi...